L25

The L25 protein seen above was determined via nuclear magnetic resonance from Escherichia coli. (Stoldt et al. 1999) Of the various proteins that interact with the 5S rRNA molecule, the role of L25 has been outlined in greatest detail. The specific area of the 5S rRNA recognized by the L25 protein is known as the E-loop, which can be seen below thanks to the crystal structure provided by Correll et al. (1997)

Within the L25 protein is a six-stranded beta barrel that can recognize the minor groove of the E-loop 5S rRNA. Binding can occur by the L25 N-terminus conjoining with the E-loop major groove and a subsequent conformational change. The role of the L25-5S rRNA interaction is unknown, although signal transduction or increased stabilization are viable possibilities. (Stoldt et al. 1999)

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