16S rRNA

Image of 16S rRNA (ribosomal proteins not shown)
16s rRNA

16S rRNA makes up the bulk of the 30S subunit. It is important for subunit association and translational accuracy. It consists of 1542 bases and contains the substrate binding A-, P-, and E- sites. The P- site is occupied by peptidyl-tRNA and is located in the major groove in an upper portion of the rRNA. The A- site is the attachment site for an incoming aminoacyl-tRNA, and is wide and shallow, which gives it a lower affinity for tRNA so it may relocate to the P- site. The E- site, occupied by deacylated tRNAs when they exit, is associated with ribosomal proteins more than the A- or P- site, perhaps facilitating the dissociation of the codon-anticodon pair during translation.

The primary structure of 16S rRNA is highly conserved. Its secondary structure is reminiscent if the clover shape of tRNA-small double helical regions are punctuated by short single stranded regions. The tertiary structure is the general shape of the whole subunit.

The arrangement of the 16S rRNA creates a 5' domain, central domain, 3' major domain, and a 3' minor domain.


5'domain
The 5' domain consists of 19 double helices that makes up the bulk of the body. It traverses a diagonal of the ribosome, when viewed from the 50S.
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5'domain
The central domain of the rRNA generates the platform and is an elongated, curved structure of nine helices, with the junction of helices 20, 21, and 22 being at the heart of it.
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3'majordomain
The 3' major domain contains 15 helical elements and composes the head.
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3'minordomain
The 3' minor domain contains 2 helices and projects from the subunit to interact with the 50S subunit.
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(Brimacombe, 1988)
(Carter, 2000)
(Malhotra, 1994)
(Wimberly, 2000)

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