The primary paper (Rice, P. A. and Steitz, T. A. (1994). "Model for a DNA-mediated synaptic complex suggested by crystal packing of [[gamma]]d resolvase subunits." EMBO J. 13, 1514-1524), to be discussed 2/7/96, concerns the development of a model for a recombinase complex involving altogether about 230 bp of DNA and 12 protein molecules (~20 kd). We are interested in the sources of the model, in comparisons with some later work, and in the biochemical implications of the structure.
Background Area (paper) Role of individual subunits (Grindley, 1993) Early topological methods applied to resolvase (Wassarman et al., 1985) Evidence for DNA shape in the complex (Salvo and Grindley, 1988) Subunit exchange vs. strand exchange models (Stark and Boocock, 1994) Functions of different monomer interfaces (Hughes et al., 1993) Evidence for unwinding/distortion (Hatfull et al., 1987) Crystal structure of complete recombinase (2 readers) (Yang and Steitz, 1995) Cooperativity mutants (Hughes et al., 1990) Large-domain crystal structure (Sanderson et al., 1990)
Grindley, N. D. F. (1993). "Analysis of a Nucleoprotein Complex: The Synaptosome of [[gamma]]d Resolvase." Science 262, 738-740.
Hatfull, G. F., Noble, S. M., and Grindley, N. D. F. (1987). "The [[gamma]]d Resolvase Induces an Unusual DNA Structure at the Recombinational Crossover Point." Cell 49, 103-110.
Hughes, R. E., Hatfull, G. F., Rice, P. A., Steitz, T. A., and Grindley, N. D. F. (1990). "Cooperativity Mutants of the [[gamma]]d Resolvase Identify an Essential Interdimer Interaction." Cell 63, 1331-1338.
Hughes, R. E., Rice, P. A., Steitz, T. A., and Grindley, N. D. F. (1993). "Protein-protein interactions directing resolvase site-specific recombination: a structure-function analysis." EMBO J. 12, 1447-1458.
Salvo, J. J. and Grindley, N. D. F. (1988). "The [[gamma]]d resolvase bends the res site into a recombinogenic complex." EMBO J. 7, 3609-3616.
Sanderson, M. R., Freemont, P. S., Rice, P. A., Goldman, A., Hatfull, G. F., Grindley, N. D. F., and Steitz, T. A. (1990). "The Crystal Structure of the Catalytic Domain of the Site-Specific Recombination Enzyme [[gamma]]d Resolvase at 2.7Å Resolution." Cell 63, 1323-1329.
Stark, W. M. and Boocock, M. R. (1994). "The Linkage Change of a Knotting Reaction Catalyzed by Tn3 Resolvase." J. Mol. Biol. 239, 25-36.
Wassarman, S. A., Dungan, J. M., and Cozzarelli, N. R. (1985). "Discovery of a Predicted DNA Knot Substantiates a Model for Site-Specific Recombination." Science 229, 171-174.
Yang, W. and Steitz, T. A. (1995). "Crystal Structure of the Site-Specific Recombinase [[gamma]]d Resolvase Complexed with a 34 bp Cleavage Site." Cell 82, 193-207.