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J Mol Biol 267 (4): 807-817 (Apr 11 1997)

Identification and characterization of genomic nucleosome-positioning sequences.

Widlund HR, Cao H, Simonsson S, Magnusson E, Simonsson T, Nielsen PE, Kahn JD, Crothers DM, Kubista M

Department of Biochemistry and Biophysics, The Lundberg Institute, Chalmers University of Technology, Goteborg, Sweden.

Positioned nucleosomes are believed to play important roles in transcriptional regulation and for the organization of chromatin in cell nuclei. Here, we have isolated the DNA segments in the mouse genome that form the most stable nucleosomes yet characterized. In separate molecules we find phased runs of three to four adenine nucleotides, extensive CA repeats, and in a few cases phased TATA tetranucleotides. The latter forms the most stable nucleosome yet characterized. One sequence with CAG repeats was also found. By fluorescence in situ hydridization the selected sequences are shown to be localized at the centromeric regions of mouse metaphase chromosomes.

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PMID: 9135113, MUID: 97280786